ES&H Manual



6650 Appendix T1

Minimum Mitigation Requirements for

Unbound Engineered Nanoparticles



1.0            Purpose


This appendix defines Jefferson Lab’s minimum mitigations requirements for engineered nanoparticles (NP). These minimum requirements are consistent with the requirements of 10 CFR 851 and Department of Energy (DOE) Order O456.1A.


2.0            Scope


This appendix addresses minimum hazard mitigations when working with engineered NPs. The process steps for this procedure are performed in coordination with ES&H Manual Chapter 6650 Unbound Engineered Nanoparticle Program.


3.0            Responsibilities

NOTE:           Management authority may be delegated at the discretion of the responsible manager.


3.1              Engineered NP Worker

·         Contact Industrial Hygiene for appropriate area and container labeling and signage requirements.

·         Contact Industrial Hygiene prior to removing any NP, including waste, from a designated area.


3.2              Industrial Hygiene

·         Annually survey and certify exhaust ventilation and vacuum cleaners used in designated NP areas.

·         Approve personal protective equipment (PPE) for NP handling.

·         Coordinate NP waste disposal.

·         Post signs on all areas designated as NP work areas.


4.0            Process Steps

Below are minimum requirements that must be incorporated into NP activities. 


4.1              Controls

Controls are evaluated using a risk-based graded approach that takes into consideration available toxicological and environmental data. The order of preference (most preferred to least preferred) for handling nanomaterial is:

1)      Solid materials with imbedded nanostructures

2)      Solid materials with nanostructures fixed to the material’s surface

3)      NP suspended in liquids

4)      Dry, unbound engineered NP, NP agglomerates, or NP aggregates


4.2              Ventilation

Work that could generate airborne unbound engineered NP is conducted in ventilated enclosures (such as a ventilation hood, glove box, or glove bag) designed to protect workers from airborne contaminants. Ventilation systems for these enclosures are protected with high efficiency particulate air filtering system (HEPA) (or Ultra Low Purifying Air, ULPA) filtration systems to “scrub” air prior to being exhausted outdoors. See ES&H Manual Chapter 6630 Appendix T1 Engineering Controls – Local and Portable Exhaust Systems for set-up and inspection requirements.

·         If the activity cannot be enclosed alternative controls such as local exhaust ventilation systems may be used to capture fugitive NP at the source.

·         Ventilation systems that can produce “blow back” such as a laminar flow hood are not used with NP.

·         Enclosed systems under positive pressure are used in a negative pressure enclosure and exhausted prior to opening.

·         Controls are in place to exclude NP from any exhausted air entering the work space; exhausted air is not re-circulated within the laboratory, even if HEPA-filtered.

·         Exhaust ventilation systems are annually surveyed (e.g., face velocity/capture velocity) to control worker exposure, and

·         HEPA filters in exhaust systems and vacuum cleaners in NP work areas are annually certified to verify filter efficiency.


4.3              Storage

Contact Industrial Hygiene prior to storage of any NP for guidance as to the proper requirements based on material composition. Minimum requirements include:

·         Affixed labels to all containers (see Section 4.4 Posting and Labeling Requirements)

·         Keep containers closed at all times while in storage

·         Store in designated areas


4.4              Posting and Labeling Requirements

4.4.1        Designated engineered NP areas (e.g., laboratory door, glove box) are posted with the following signage:



4.4.2        All engineered NP storage containers are labeled:


4.5              Personal Protective Equipment (PPE)

PPE is selected per specific NP. PPE requirements are documented in applicable Operational Safety Procedure or Task Hazard Analysis for a specific NP activity.


4.6              Waste Management

NPs are managed as a hazardous waste in accordance with ES&H Manual Chapter 8061 Hazardous Waste Management. NPs are NOT released to the sanitary sewer or disposed of in regular trash bins.


All waste (including wipes, filters, gloves, etc.) that has been in direct contacted with NPs is wetted, bagged, and placed in a designated Satellite Accumulation Area (SAA) or contact Industrial Hygiene for immediate removal.


4.7              Housekeeping

Surfaces where NPs are used are cleaned at the end of each work-shift using approved HEPA vacuum and wet-wiping.

·         A HEPA vacuum (approved by Industrial Hygiene) is required in work areas where NPs are used.

·         Used wipes are disposed of in designated SAA.


4.8              Transport Requirements

Jefferson Lab treats engineered NP as suspect carcinogens and as such are considered hazardous material. Minimum requirements for off-site shipment are provided within ES&H Manual Chapter 6660 Appendix T1 Transport of Hazardous Material. Additional requirements include:


4.8.1        Documentation and Notifications

·         Available descriptions of the material (e.g., Safety Data Sheets (SDSs)). Researchers prepare a document that describes properties that are reasonably likely to be exhibited by samples.

·         A notification to receiving facility of the incoming shipment.


4.8.2        Transport by Personal Vehicle

·         Driver must have a valid driver’s license

·         Vehicle is in good mechanical condition with valid state inspection sticker

·         Vehicle has minimum liability insurance required by the state

·         Driver obeys all traffic rules


NOTE: Only shipments of NP classified as “other materials” NOT recognized as DOT hazardous materials, are transported by personnel vehicle.


5.0            Revision Summary


Revision 1.0 – 08/17/16 – Periodic Review; formerly titled ‘Minimum Mitigation Requirements for Engineered Nanoparticles’; updated per DOE Order 456.1A  

Periodic Review – 06/28/13 – Currently no nanoparticle activity; no changes per TPOC

Revision 0.0 – 04/17/09 – Initial content  











ES&H Division

Jennifer Williams





This document is controlled as an on line file.  It may be printed but the print copy is not a controlled document.  It is the user’s responsibility to ensure that the document is the same revision as the current on line file.  This copy was printed on 8/23/2016.